A Research Program for Identification and Characterization of Protein Complexes
The Genomic Science Center for Molecular and Cellular Systems (CMCS) performs experimental measurements of protein-protein interactions in microbial species. Because most proteins perform their function in concert with other proteins, knowledge of interactions among proteins is an important component of systems biology. This website provides access to our results, and to tools for using these results.
- Support for the CMCS is through the U.S. Department of Energy Genomic Science program, which seeks insight into fundamental biological processes through genome-scale measurements and predictions. The Genomic Science program aims to use this knowledge to harness the diversity of microbial life to applications for energy and the environment.
- Our measurement strategy is based on affinity isolation of targeted proteins and the proteins with which they interact, followed by mass spectrometric identification of these proteins.
The current focus of the CMCS is the bacterial species Rhodopseudomonas
palustris. Our current throughput is 16 target proteins per week.
projects aimed at eliminating bottlenecks have a common goal of doubling
our throughput each year through 2010. At this rate, we anticipate
completing our study of interactions among soluble cytosolic proteins
in R. palustris by mid 2008.